重组人RSV-F (RSVgp08)蛋白

产品货号#:

11049-V08B

规格(价格)

X

收藏该产品

Human respiratory syncytial Virus Protein Datasheet

RSVgp08蛋白价格信息

50ug: 询价;

200ug: 询价;

≥1mg 大包装: 询价

RSVgp08蛋白产品信息

RSVgp08 别名:

F, HRSVgp08

表达方式:

A DNA sequence encoding the extracellular

domain of human RSV Fusion glycoprotein (AAB59858.1) (Met 1-Thr 529) was

expressed, fused with a polyhistidine tag at the C-terminus.

种属:

Human respiratory syncytial Virus

表达宿主:

Insect cells

RSVgp08蛋白质控指标

纯度:

> 95%, as determined by SDS-PAGE

SDS-PAGE:

内毒素:

< 1.0 EU per 1μg of the protein as determined by the LAL method.

稳定性:

Samples are stable for up to twelve months from date of receipt at -70°C

预测N端:

Phe 22

分子量:

The secreted recombinant human RSV-F comprises

518 amino acids with a predicted molecular mass of 58 kDa. As a result

of highly glycosylation, it migrates as an approximately 65-75 kDa band

in non-reduced SDS-PAGE.

缓冲液:

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0, 10% glycerol

RSVgp08蛋白使用指南

储存方法:

Store it under sterile conditions at -70°C.

It is recommended that the protein be aliquoted and be used as soon as

possible. Avoid repeated freeze-thaw cycles.

Reconstitution:

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RSVgp08 Protein Related Products &Topics

相关研究领域:

Virus Research Tools>>RSVgp08

蛋白:

抗体:

RSVgp08蛋白背景综述

Human respiratory syncytial virus (HRSV) is the most

common etiological agent of acute lower respiratory tract disease in

infants and can cause repeated infections throughout life. It is

classified within the genus pneumovirus of the family paramyxoviridae.

Like other members of the family, HRSV has two major surface

glycoproteins (G and F) that play important roles in the initial stages

of the infectious cycle. The G protein mediates attachment of the virus

to cell surface receptors, while the F protein promotes fusion of the

viral and cellular membranes, allowing entry of the virus

ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV

is synthesized as a nonfusogenic precursor protein (F0), which during

its migration to the cell surface is activated by cleavage into the

disulfide-linked F1 and F2 subunits. This fusion is pH independent and

occurs directly at the outer cell membrane, and the F2 subunit was

identifed as the major determinant of RSV host cell specificity. The

trimer of F1-F2 interacts with glycoprotein G at the virion surface.

Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is

unmasked and induces the fusion between host cell and virion membranes.

Notably, RSV fusion protein is unique in that it is able to interact

directly with heparan sulfate and therefore is sufficient for virus

infection. Furthermore, the fusion protein is also able to trigger

p53-dependent apoptosis.

参考文献

Martin-Gallardo, A. et al., 1993, J Gen Virol. 74 ( Pt 3): 453-458.

Jose, A. M. et al., 1997, J Gen Virol. 78: 2411-2418.

Feldman, S.A. et al., 1999, J Virol. 73 (8): 6610-6617.

Zlateva, K. T. et al., 2004, J Virol. 78 (9): 4675-4683.

Trento, A. et al., 2006, J Virol. 80 (2): 975-984.

Branigan, P. J. et al., 2006, J Gen Virol. 87 (Pt 2): 395-398.

Eckardt-Michel, J. et al., 2008, J. Virol. 82: 3236-3249.