Influenza A H1N1 (A/California/07/2009) Hemagglutinin

产品货号#:

11085-V08H

规格(价格)

X

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Influenza A H1N1 ( A/California/7/2009 ) Hemagglutinin / HA Protein

H1N1 ( A/California/7/2009 ) HA Protein Price Inquiry ( Available Sizes )

500 μg: 询价;

≥1mg 大包装: 询价

H1N1 ( A/California/7/2009 ) HA蛋白产品信息

别名:

HA

表达方式:

A DNA sequence encoding the extracellular domain of

Influenza A virus H1N1 (A/California/07/2009) (ACP44189.1)

hemagglutinin（Met 1 - Gln 529 ）( HA1+HA2, two amino acids deletion,

uncleaved ) was fused with a polyhistidine tag at the C-terminus

种属:

Influenza A Virus H1N1

表达宿主:

Human Cells

H1N1 ( A/California/7/2009 ) HA Protein QC Testing

纯度:

> 97 % as determined by SDS-PAGE

SDS-PAGE:

H1N1 HA protein

内毒素:

< 1.0 EU per μg of the protein as determined by the LAL method.

稳定性:

Samples are stable for up to twelve months from date of receipt at -70°C

预测N端:

Asp 18

分子量:

The secreted recombinant hemagglutinin of Influenza A

virus H1N1 (A/California/07/2009) comprises 521 amino acids with the

predicted molecular mass of 59 kDa. As a result of glycosylation, it

migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing

conditions

缓冲液:

Lyophilized from sterile PBS , pH 7.4

H1N1 ( A/California/7/2009 ) HA Protein Usage Guide

储存方法:

Store it under sterile conditions at -70°C upon

receiving. Recommend to aliquot the protein into smaller quantities for

optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution:

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

H1N1 HA蛋白背景综述

Hemagglutinin (HA) is a single-pass type I integral membrane

glycoprotein from the influenza virus, and comprises over 80% of the

envelope proteins present in the virus particle. In natural infection,

inactive HA is matured into HA1 and HA2 outside the cell by one or more

trypsin-like, arginine-specific endoprotease secreted by the bronchial

epithelial cells. Binding of HA to sialic acid-containing receptors on

the surface of its target cell brings about the attachment of the virus

particle to the cell and forms a endosome. Low pH in endosomes induce an

irreversible conformational change in HA2, releasing the hydrophobic

portion “fusion peptide”. After which, virus penetrates the cell and

pours its contents including the RNA genome into the cytoplasm mediated

by fusion of the endocytosed virus particle’s own membrane and the

endosomal membrane. Hemagglutinin plays a major role in the

determination of host range restriction and virulence.

H1N1 ( A/California/7/2009 ) HA Related Influenza Virus Research Tools

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H1N1 ( A/California/7/2009 ) HA Protein Related Products & Topics

H1N1 HA Protein Related Areas:

相关蛋白:

相关抗体:

参考文献

Günther-Ausborn, S. et al., 2000, J. Virol. 74: 2714-2720.

Hoffman, LR. et al., 1997, J. Virol. 71: 8808-8820.

Marjuki, H. et al., 2006, J. Biol. Chem. 281: 16707-16715.

Gething, M-J. et al., 1980, Nature. 287: 301-306.

Naeve, C.W. and Williams, D. 1990, EMBO. J. 9: 3857-3866.