重组人Human FCGRT & B2M Heterodimer(His Tag)蛋白

产品货号#:

CT009-H08H

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Human FCGRT & B2M Heterodimer Protein

Human FCGRT & B2M Heterodimer Protein Price Inquiry ( Available Sizes )

200μg: 询价;

≥1mg 大包装: 询价

Human FCGRT & B2M Heterodimer蛋白产品信息

Protein Name :

Heterodimer of IgG receptor FcRn large subunit p51 (

alpha chain ) ( FCGRT ) and Beta-2-microglobulin ( beta chian, subunit

p14 ) ( B2M )

Type:

Human FCGRT & B2M Heterodimer Protein

表达方式:

A DNA sequence encoding the extracellular domain ( Met 1

- Ser 297 ) of human FCGRT ( P55899 ) was fused with a polyhistidine

tag at the C-terminus, constructed the plasmid 1; A DNA sequence

encoding the human B2M ( P61769 ) ( Met 1 - Met 119 ) constructed the

plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M

heterodimer was purified

种属:

Human

表达宿主:

Human Cells

Human FCGRT & B2M Heterodimer蛋白质控指标

纯度:

> 95 % as determined by SDS-PAGE

SDS-PAGE:

FCGRT & B2M protein

内毒素:

< 1.0 EU per μg of the protein as determined by the LAL method

稳定性:

Samples are stable for up to twelve months from date of receipt at -70°C

预测N端:

Ala 24 & Ile 21

分子量:

The recombinant heterodimer of human FCGRT / B2M

comprises 385 ( 285 + 100 ) amino acids and has a calculated molecular

mass of 43.5 ( 31.8 + 11.7 ) kDa. The apparent molecular mass of rh

FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in

SDS-PAGE under reducing conditions

缓冲液:

Lyophilized from sterile PBS , pH 7.4

Normally 5 % - 8 % trehalose and mannitol are added

as protectants before lyophilization. Specific concentrations are

included in the hardcopy of COA.

Please contact us for any concerns or special requirements.

Human FCGRT & B2M Heterodimer蛋白使用指南

储存方法:

Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution:

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Human FCGRT & B2M Heterodimer蛋白相关产品和研究主题

相关领域:

Proteins:

Antibodies:

Human FCGRT & B2M Heterodimer蛋白背景综述

IgG receptor FcRn large subunit p51, also known as IgG Fc fragment

receptor transporter alpha chain, Neonatal Fc receptor, FCGRT and FCRN,

is a single-pass type I membrane protein which belongs to

the immunoglobulin superfamily. FcRn / FCGRT is a MHC class I like

molecule that functions to protect IgG and albumin from catabolism. FcRn

/ FCGRT binds to the Fc region of monomeric immunoglobulins gamma. It

mediates the uptake of IgG from milk. It may play a role in transfer of

immunoglobulin G from mother to fetus.

Beta-2-microglobulin, also known as B2M, is a secreted protein which

belongs to the beta-2-microglobulin family. It contains one Ig-like

C1-type (immunoglobulin-like) domain. Beta-2 microglobulin (B2M) plays a

pivotal role in the biology of mammals. B2M forms the small invariable

light chain subunit of class I HLA antigens on the cell membrane of

all nucleated cells. During the continuous turnover of the HLA

molecules. B2M is shed from the cell membrane into blood. Lymphocytes

are the main source of serum free B2M. It associates not only with the

alpha chain of MHC class I molecules, but also with class I-like

molecules. B2M is necessary for cell surface expression of MHC class I

and stability of the peptide binding groove. In fact, in the absence of

B2M, very limited amounts of MHC class I (classical and non-classical)

molecules can be detected on the surface.

FcRn complex consist of two subunits: p51, and p14 which is equivalent

to beta-2-microglobulin (B2M). It forms an MHC class I-like

heterodimer. B2M is a component of the class I major histocompatibility

complex (MHC). B2M is also an integral component of the FcRn

heterodimer. Failure of passive transfer (FPT) is a condition in which

neonates do not acquire protective serum levels of maternal antibodies.

A principal component of antibody transport is the neonatal receptor

for the Fc portion of immunoglobulin, a heterodimer of a MHC-1

alpha-chain homolog ( FcRn ) and beta-2-microglobulin ( B2M ).

参考文献

He X.H., et al., 2004, Sheng Wu Gong Cheng Xue Bao. 20: 99-103.

Clawson,M.L. et al., 2004, Mamm Genome. 15 (3):227-36.

Kihara M., et al., 2006, J. Biol. Chem. 281: 31061-31069.

Ricagno S.,et al., 2009, Biochem.Biophys.Res.Commun. 380: 543-547.

Kuo,T.T. et al., 2010, J Clin Immunol 30 (6):777-89.